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1. DESIGN IT

1.1 Structure Based design

1.1.1 Drug design - Ligand Binding optimisation

1.1.2 Drug Design - Virtual Screening

1.1.3 Drug Design - Epitope Mapping Strategy

1.1.4 Modeling for Protein Engineering : Solubility/Aggregation State

1.1.5 Modeling for Protein Engineering : Stability

1.1.6 Modeling for Protein Engineering : Substrate Specificity

1.1.7 Modeling for Protein Engineering : rate of Catalysis

1.1.8 Modeling for Antibody Humanisation

1.1.9 Modeling of Fusion Proteins

1.2 Gene Construct Design & Synthesis

1.2.1 Transcription and Translation Optimization

1.2.2 Post Translational Modifications

1.2.3 Unnatural Amino Acid Incorporation

1.2.4 Host Cell Compartment Targeting

1.2.5 Host Cell Considerations

1.2.6 Rapid and Cost Effective Gene Synthesis

2. MAKE IT

2.1 Protein Production and Downstream Processing

2.2 Formulation

2.1.1 Cell Culture : E. coli, yeast, insect & mammalian cells, bacterial host other than coli

2.1.2 Cell culture capabilities : Cell growth & expression optimization, up to 100 Liters

2.1.3 Extraction & purification capabilities, up to 100 L scale

2.1.4 Biochemical & biophysical characterization : Standard package (Electrophoretic techniques, LC-MS, Sequencing, DLS, functional assays)

2.1.5 Biochemical & biophysical characterization : Customized biophysical package (CD, Thermal denaturation profile, NMR & X-ray)

2.3 Conjugation

2.2.1 Sparse matrix approach to buffer & excipient identification

2.2.2 Lyophilization & shelf life determination

3. TEST IT

3.1 Expression Testing & Optimization

3.2 Functional Assay Development

3.2.1 Enzyme Screening Assays

3.2.2 Receptor Binding Screening Assays

3.2.3 HTS Customozation

3.3 Hit Validation based on Biophysical Techniques

3.3.1 High throughput thermal denaturation analysis

3.3.2 Isothermal Calorimetry

3.3.3 LC-MS